Monday, January 27, 2020

Sequence Analysis East Of Eden Film Studies Essay

Sequence Analysis East Of Eden Film Studies Essay Elia Kazans film East of Eden attempts to explore the relationship between father and son, Adam and Cal. Cal is desperately seeking love from his father, but he just turns away from Cal and leaves him upset. Conflicts arise between the two and this tears them further apart from each other. In the sequence, Kazan applied non-diegetic elements and various visual techniques to show the tension between Adam and Cal. Apart from that, Kazan also applied different mise-en-scene arrangements like lighting and blocking to reflect the emotions of each character and to illustrate Cal feels distant from his father and bring up the problems caused by the generation gap. With the use of all these film techniques, Kazans film has also demonstrated an understanding of the classical Hollywood style at that time. Kazan has used non-diegetic music to create suspicion in the film, and particular camera angles to reflect the bond Cal and Adam. Within the clip, it can be seen that aggressive music is used when Cal is telling Aron that their mother is still alive. The director used music as a way to illustrate the conflicts between the brothers, which are significant to the film narration. Burt suggested that this is the climactic scene in the film, and the music has successfully filled the explosive potential of the story plot (1994, p.185). Non-diegetic music has been extensively used in different parts of the film, and the repetitive use of music helps illustrate the emotions of the characters to the audience. In the clip, complex harmonies and thudding chordal figure are used to bring out the explosive nature of the climax scene (Burt, 1994, p.186). With the use of background music and lightings in the clip, the director has been able to bring suspense to the audience, and it becomes a clue to what will happen next. At the same time, non-diegetic music has been used for the entire sequence of Sunrise (1927). Due to the silent nature of this film, the emotions of the characters cannot be brought out through the tones of dialogues. In that case, storytelling has to rely on the use of orchestral music. Also, the director applied a canted framing, which means a slope is formed within the frame (Bordwell and Thompson, 2010, p.490). Kazan used this technique to capture the scene when Cal left heartbroken after being rejected by his father. A similar slanted camera angle has been used at the beginning of the film when Cal is trying to confront his father about his mothers death. It can be seen that when the tension between Cal and Adam arises, the director used a canted frame to capture the scene, as a leitmotif to show the distrust between the two throughout the film. The slope could be seen a metaphor of their relationship, indicating that their relationship is unstable. Mise-en-scene arrangements are also evident in the clip. Kazan has put different components into different scenes and he arranged them in a meaningful way. In terms of blocking, it can be seen that Cal often stands further away from other characters and the camera. For instance, in the scene when Cal tries to explain how he got the money to Adam, they stand at diagonal corners, with Adam standing closer to the camera. In an interview, Kazan explained that Cinemascope was used to put Cal at the corner of the frame so that he would look tinier than other characters (Young, 2001, p.203). This technique is used throughout the film in order to show that he is powerless in front of his father. Similar settings can also be seen when Cals brother is confronting with him, a medium shot of Aron standing in front of Cal. After that, it switches to a long-shot, and we could only see the shades of the two characters. Burt suggested that the light settings have made their appearances barely recogn isable (1994, p.188). The darkness has created a feeling of fear in the film, and the staging of the scene has also shown Cals emotional changes, from frustration to anger. As mentioned, the director often put Cal at the corner as he was portraying him as a kid (Young, 2001, p.203). However, when Cal is confronting his brother, he stands on the bench under the tree, indicating a regain of power. At that point, it demonstrates a mental change in Cal, as he is trying to act maturely. The sequence has also illustrated the dominant features of the classical Hollywood film style. According to King, classical style is analysed in two aspects, film narrative and editing techniques (2002, p.4). A typical Hollywood film usually shows how the main character finds a solution to his problem (Bordwell, 1986, p.18). In terms of narrative, East of Eden presents the story in a linear timeline, with Cal struggling to maintain a healthy relationship with his father. It can be seen that the sequence leads audience to the climax of the story plot, as Cal faces an obstacle when he is attempting to resolve his own issues, thus he expresses his long hidden jealousy towards his brother, indicating a breaking change in the relationships between each character. Therefore, the sequence fulfils the struggles for problem solving part of the Hollywood film plot, as suggested by Bordwell (1986, p.18). In the clip, it can also be seen that the director applied continuity editing. This editing method involves point-of-view shot and shot/reverse-shot, which has been used since the 1910s (Bordwell, Straiger, Thompson, 1985, p.194). An example of the point-of-view shot is that after a medium shot that shows Adam asking Cal how he got his money, Cal looks at Abra, and a medium shot of her is shown. The camera is placed at Cals visual direction, and it shows that Cal is getting courage from Abra. Meanwhile, throughout the movie, shot/reverse-shot is often used during conversations between characters. This gives a clear view of each character, and it allows audience to see their facial expressions clearly, which is an important element of a melodrama. Maltby suggested that James Dean in East of Eden was a method performance that combines psychoanalysis and melodrama, which constructs realism and showing expressive emotions at the same time (1995, p.263). Compared with Sunrise, James Dean has a more natural performance. Because of the film nature, actors in Sunrise have to exa ggerate their expressions to illustrate the whole story. In conclusion, that sequence in the film East of Eden has brought the story plot to the climax point, with the use of various visual techniques and scene arrangements. Non-diegetic music and canted frame can be found in the sequence, which are used to build up the tension between Cal and other characters. In addition, Kazan has used staging and lighting subtly to reflect the feelings of each character. Classical Hollywood styles like continuity editing and exaggerated acting can also be seen in the sequence, which are the main features of the Hollywood films at that time. The techniques that are used in the sequence can also be found in other parts of the movie, and this shows that they are essential in constructing the whole structure of the film.

Sunday, January 19, 2020

Pre-Transfusion Blood Tests

Pre-transfusion Blood Tests: Title: To perform the following tests: ABO and RH grouping using the Diamed Gel Card system. Rh and Kell phenotyping (antigen typing) using the Diamed Gel Card system. Direct Coombs Test (DCT) using the conventional tube system. Direct Coombs Test (DCT) using the Diamed Gel Card system. Antibody Identifications (IAT) technique using the conventional tube system. Antibody Identifications (ETC) technique using the Diamed Gel Card system. Name: S. Ward Date: 8/11/2012 Introduction: The objective of this practical is to perform some pretransfusion tests, using various methods.Pretransfusion testing is carried out in all hospital blood bank laboratories and is used to minimise the risk of encurring a haemolytic transfusion reaction. Haemolytic transfusion reactions occur when a patient is transfused with red cells which have a foriegn antigen on the cell surface that the patient has an antibody to. While the ABO and Rh blood group systems are the most antigeni c and thus can cause severe haemolytic transfusion reactions. The other blood group systems can also cause a (less severe) haemolytic reaction, these reactions can be fatal and so the procedures to avoid them are of great importance.Materials & Method: Not all tests were performed by all members of the class. ABO and Rh blood grouping using the Diamed Gel Card system; performed. Rh and Kell trying using the Diamed Gel Card system; not performed. Direct Coombs Test (DCT) using the Diamed Gel Card system;not performed. Antibody Identification (ETC) technique using the Diamed Gel Card system; performed but not centrifuged so no results were obtained. Antibody Identifications (IAT) technique using the conventional tube system; performed Direct Coombs Test (DCT) using the conventional tube system; erformed. Cell 1% suspensions were prepared for the bench from a 3% cell suspension provided. Results: As well as some tests not being completed by everyone, some results were not obtained as t here was a queue for the ID centrifuge. Results below are ones obtained by myself; ABO & Rh grouping; Known O+ cells were tested. Expected results would be; However, the gel matrix had dried out so no results were obtained. Antibody Identifications (IAT) technique using the conventional tube system; + + + – – + – – + +When these results are compared with the ID panned profile, its is seen that there is no antigen which matches the antibody in the patient's plasma. Traditional Direct Coombs test results was positive. Discussion: The results from this practical were not as expected. With regard to the ABO Rh typing, the expected result for the O+ blood tested is as shown above. The reason why the results obtained were incorrect were because the gel had dried out. This shows that it is very important to ensure that the reagents used in the transfusion laboratory are of a really high quality to ensure that all results obtained are reliable.With regard to the traditional antibody profile, it is seen that there is no antigen which matches the antibody in the patient's plasma according to the ID panel. It is possible that the patient has an uncommon antigen which isn't on the ID panel. However, it's more likely that there was human error in labelling the reaction tubes 1-10. The direct coombs test checks to see in vivo sensitisation to IgG antibodies. The traditional coombs test results for this practical showed aggultenation when treated with antihuman globulin, which is a positive result.Results for the other laboratory tests using Diamed Gel Card system were unobtained due to there being a queue for the centrifuge. A brief explanation of all these tests is explained below; ABO & Rh D grouping; this can be done by conventional tube technique, as performed previously, or can be done using the Diamed Gel Card system. These gel cards contain known antobodies on a gel matrix. A positive result shows the red cells kept at the top of the gel m atrix, a negative result sees the red cells going down through the matrix to the bottom of the card.This has replaced the traditional method as it allows for automation. Rh & Kell phenotyping; this is done using the Diamed Gel Card sytem and is similiar to the ABO and Rh typing method. This shows which, if any of the main Rh or Kell antigens are on the patients red cells. The results are read in the same manner as the ABO and Rh D as described above. Direct Coombs Test using the conventional tube system; The direct antiglobulin test is used to detect in-vivo sensitisation and detects small IgG antibodies on a patient's red cells.The conventional tube method involves washing the cells three times for one minute, resuspending each time, then finally adding two drops of antihuman globulin and centrifuge once more for 20 seconds and results were recorded. This process is laborious and so can be replaced with the next method. Direct Coombs Test using the Diamed Gel Card system; The gel c ard system involves the addition of 1% cell suspension of test cells to the Anti IgG card, this is centrifuged for 10 minutes and results are recorded. This method also shows in-vivo sensitisation and detects small IgG antibodies on the patient's red cells.Antibody Identifications (IAT) technique using the conventional tube system; Antibody identification is used as a follow-up test to a positive indirect antiglobulin test. The antibody identifcation test is used to determine the red cell antibodies in the patient's plasma. If one or more clinically significant red cell antibodies are identified, then donor blood that lacks the corresponding red cell antigens must be used for tranfusion, this is reffered to as antigen negative blood. The conventional tube system involves reacting the patients plasma with the 10 commercial identification cells.This is incubated for 30 minutes at 37Â °c, each of the reaction tubes are washed 3 times for one minute, resuspended after each time and the n two drops of antihuman globulin are added and the tubes are centrifuged for an additional 20 seconds and results are recorded. This process is laborious, and so can be replaced by the gel card system which can be automated. Antibody Identifications technique using the Diamed Gel Card system; The method is the similiar to the other gel card systems and the principle it the same as the other antibody identification.As mentioned bofore, this process allows for automation and so is favoured in large labs. Questions: Principle of the Direct and Indirect Coombs test: The principle of the Coombs' test is that addition of rabbit anti-human IgG to the patient's blood will result in aggregation of the patient's red cells if the red cells are coated in small IgG antibodies that will not agglutenate normally at room temperature. So, the direct antiglobulin test is used to detect in-vivo sensitisation to IgG antibodies. Applications:The direct coombs test is udes to test for autoimmune haemoly tic anaemia. The indirect coombs test can be used to detect very small amounts of antibodies present in a patients plasma and if used in antenatal care to screen pregnant women for antibodies that may cause haemolytic disease of the newborn. It can also be used for compatability testing, antibody identification, RBC phenotyping and titration studies. Controls: The positive control is sensitised O+ cells and the negative control is O- cells.

Friday, January 10, 2020

New York MTA

Traditional market structure suggests that all market decisions should be based on utilitarian theory. We often witness market decisions which neglect other important aspects of the market activity. As a result, we appear under the impact of one-side unbalanced decisions which ultimately neglect the principles of morality and moral theology of the marketplace.Rising fares and tolls by MTA  Ã¢â‚¬Å"After an unusually vigorous and spirited debate, the board of the Metropolitan Transportation Authority voted to raise fares on subways, buses and commuter railroads and tolls on bridges and tunnels† (Chan, 2007a). Why is it so surprising that not all members of the MTA board wanted to turn into the proponents of fares and tolls’ increase? Does this mean that more and more political and business players realize the importance of morality in taking market decisions?Evidently, the situation is much worse than one may imagine. One may initially think that increasing the fares wil l lead to less traffic congestion, and will urge more people to use public transport; yet, the public transport fares are being raised, too. From the viewpoint of those who vote for raising fares and tolls in New York, this decision is the first step towards â€Å"fiscal responsibility. The authority had for long applied windfalls and real estate taxes hoping that someone would bail us out and turning a blind eye to our responsibility to put this MTA on a firm future monetary structure† (Chan, 2007a).Simultaneously, from the viewpoint of morality and theology of the marketplace, commercial activity is not limited by rational market decisions, but also â€Å"confronts us with the moral predicaments† (Gregg, 2004). The major concern within this situation is that the decision to raise fares has completely neglected the position of those whom we traditionally consider to be vulnerable populations. The representative of Working Families Party is confident that raising fares will seriously hit working people (Benjamin, 2007). â€Å"Today, once again middle class New Yorkers and those struggling to make it, are bearing the cost†, Rep. Anthony Weiner said (Benjamin, 2007).â€Å"A fare hike now is the wrong choice for New York. It would hit many people who are struggling hard to make ends meet and hurt the region's economy. [†¦] This fare hike will hit 86 percent of the riding public who use fare discounts. These include pay-per-ride bonus MetroCards and 7- and 30-day unlimited-ride passes. It's also a double whammy for most L.I.R.R. and Metro-North commuters whose railroad fares would go up!† (Chan, 2007b)The discussed fare hike will also cause the bonuses' decrease for riders (from 20 to 15 percent), and the discounted fare will cost $1.74 instead of $1.67 (Chan, 2007b). The problem is that New Yorkers pay more than they have to for the transport they use. â€Å"In 2005, riders paid 55 percent of the costs of running the subways and buses† (Chan, 2007b). Objectively, this is much higher that the riders in other cities pay: those in Boston do not compensate more than 29 percent of the discussed costs, and those in Philadelphia pay no more than 37 percent (Chan, 2007b).As the M.T.A reports $140 million reductions, does this mean that they will make the riders pay this amount through higher fares and tolls? Doubtlessly, the suggested fares and tolls increase will help compensate the under-financing of the MTA by the state Government, but if the decision framework remains unchanged, this compensation will actually take place for the account of the already mentioned vulnerable populations. â€Å"To rely upon utilitarianism as the moral – philosophical foundation of the case for the market creates tremendous difficulties for Catholics† (Gregg, 2004).The utilitarian desire to find the greatest good and to satisfy the masses does not meet the ethical and moral criteria of religion. Those who were ta king the decision to raise the fares and tolls in New York have neglected one essential aspect in their decision making: when one looks for the means to produce the greatest pleasure for the greatest number of people, one has to perform numerous calculations and to produce the decision which satisfies everyone. From the viewpoint of moral theology, such calculations in market decision-making are simply impossible. â€Å"No person can make such an assessment without admitting a tremendous degree of ignorance about all the possible effects that might proceed from a freely chosen act† (Gregg, 2004).The MTA governors have evidently gone beyond their reasonable abilities, trying to persuade us that that the future with raised fares and tolls for everyone was better than other possible alternatives. The MTA board members view the increased tolls and fares as the means to close the gaps in MTA’s budget and to provide safe and reliable system of transportation for the New York ’s citizens. However, it is not the ultimate goal for those who use public transport and belong to vulnerable layers of the city population.ConclusionThe moral theology of marketplace rejects any uniform measures in defining the goals of decision making. This is why the governors should have considered the financial opportunities of those who cannot afford paying more for using public transport. The diversified structure of prices would resolve all moral and ethical issues, and would not create serious obstacles on the way towards better functioning of the city’s transportation systems.ReferencesBenjamin, E. (2007). MTA fare hike reactions (updated). Daily News. Retrieved February 17,2008 from http://www.nydailynews.com/blogs/dailypolitics/2007/12/mta-fare-hike-reactions.htmlChan, S. (2007a). Board approves subway and bus fare increase. The New York Times.Retrieved February 17, 2008 from http://cityroom.blogs.nytimes.com/2007/12/19/mta-board-approves-fare-and-toll-incr eases/index.html?hpChan, S. (2007b). Hundreds stranded online by botched M.T.A. â€Å"Webinar†. The New YorkTimes. Retrieved February 17, 2008 from http://cityroom.blogs.nytimes.com/2007/12/10/mixed-reaction-to-new-mta-fare-plan/?hpGregg, S. (2004). Ethics and the market economy: Insights from Catholic moral theology.IEA Economic Affairs, June, pp. 4-10.

Thursday, January 2, 2020

The Scarlet Letter, by Nathaniel Hawthorne - 827 Words

Why are human beings tempted to conceal their transgressions? Is it for the fear of punishment or the loss of one’s standing with the public? In the insightful novel The Scarlet Letter, a seventeenth-century Boston minister named Arthur Dimmesdale committed, in the eyes of the townsfolk, the most evil of sins: adultery. Unlike his partner in this offense, Hester Prynne, he did not accept responsibility for his crime; instead he veiled his infraction of the Puritan law from the populace of Boston. As a consequence of his attempt to hide the truth, Minister Dimmesdale felt the guilt course through him, and that inner feeling of remorse caused his health to decline, his speeches to feel hypocritical, and his belief in the Lord’s mercy to†¦show more content†¦However, she bore the punishment with open arms, hoping clean her soul of its impurity. On the other hand, Arthur Dimmesdale concealed his immense wrongdoing from the people, and as a result, continued to rece ive passionate admiration for his perceived holiness and inspiring speeches about the works of God (119). Whereas Hester’s punishment was given through the burning stares at her scarlet letter, Dimmesdale’s scarlet letter was in his heart. This guilt took hold of his life in a way that he could never escape until his death, and his health failed accordingly. In his futile attempts to absolve himself of sin, Minister Dimmesdale punished himself with late prayers, and he fasted until he could barely stand (141). In order to hide his offense, Mr. Dimmesdale suffered a fate worse than death: having to deal with this enormous guilt during every moment. Although both suffered greatly in the recent aftermath of his/her crime, Hester’s situation improved tremendously afterwards. The citizens of Boston, impressed by her humble nature and good deeds, began to stop staring at her scarlet letter and making the harsh comments about her. In fact, members of the town would tel l outsiders proudly about her kindness and helpfulness (157). Also, she was ordained a Sister of Mercy: a position that would not have had been considered in the first few years of her suffering. By sympathizing and helping otherShow MoreRelatedThe Scarlet Letter By Nathaniel Hawthorne1242 Words   |  5 PagesLYS PAUL Modern Literature Ms. Gordon The Scarlet Letter The scarlet letter is book written by Nathaniel Hawthorne who is known as one the most studied writers because of his use of allegory and symbolism. He was born on July 4, 1804 in the family of Nathaniel, his father, and Elizabeth Clark Hathorne his mother. Nathaniel added â€Å"W† to his name to distance himself from the side of the family. His father Nathaniel, was a sea captain, and died in 1808 with a yellow fever while at sea. 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